Amino acid exchanges at 40–60% of Bacillus subtilis Lipase A positions improve organic solvents resistance


Congratulations to Victorine Josiane Frauenkron‑Machedjou on her recent Publication!

Improvements for enzymes in ionic liquids Copyright: Bio VI Improved features of enzymes in ionic liquids

In Josiane´s new publication, protein engineering has been employed to successfully improve organic solvent resistance of Bacillus subtilis Lipase A (BSLA). A systematic study covering the full natural diversity of Bacillus subtilis Lipase A with one amino acid exchange was performed to explore nature’s potential to improve organic solvent resistance of enzymes. The co-solvent resistance against 2,2,2‑trifluoroethanol, 1,4-dioxane, and dimethyl sulfoxide were improved by 41 to 59% of Bacillus subtilis Lipase A positions with a total of 4–10% of all possible substitutions. Charged substitutions were preferred to improve 1,4-dioxane and 2,2,2‑trifluoroethanol resistance whereas polar ones were preferred for dimethyl sulfoxide. Charged substitutions on the surface predominantly improved resistance, polar substitutions were preferred in buried regions and 58–93% of beneficial substitutions led to chemically different amino acids. This work was financially supported by Deutsche Forschungsgemeinschaft through the Research Training Group BioNoCo—Biocatalysis using non‑conventional media GRK 1166 and the Hundred Talents Program of Chinese Academy of Sciences to Prof. Leilei Zhu.

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Frauenkron‑Machedjou, V. J., Fulton, A., Zhao J., Weber, L., Jaeger, K. E., Schwaneberg, U. and Zhu, L.: Exploring the full natural diversity of single amino acid exchange reveals that 40–60% of BSLA positions improve organic solvents resistance; Bioresour. Bioprocess. (2018) 5:2